BIOCHEMISTRY

• Biochemistry (Cell free assays on following platforms)
  • TR-FRET (HTRF)
  • Luminescence GLO (Promega)
  • Enzyme Coupled assay
  • Mode of inhibition studies (Competitive/Non-competitive inhibition)

CELL BIOLOGY

• Cell based assays [primary cells (mouse splenocytes & human PBMC’s) and cell lines]
  • Proliferation assay / Viability / Cell death assay
  • Target engagement/Mechanism of action ( Western Blotting /ELISA)
  • Immuno-oncology response - Cytokine release assays in primary cells and cell lines (ELISA)
  • Drug combination assay & determination of CI value (SYNERGY)
  • Development and characterization of resistant cell lines (Mechanism of resistance )

 

ADME

In Vitro Metabolism

• Microsomal Stability
• S9 fraction Stability
• UGT Inhibition
• Cytochrome P450 Inhibition
• Metabolite Profiling and Identification
• Plasma Stability

Non-CYP mediated Metabosim

• Aldehyde Oxidase Metabolism

Protein Binding

• Plasma Protein Binding

Bio-Analysis

• BioAnalytical Method development and quantification

Solubility & Stability Studies

• Kinetic Solubility
• Thermodynamic Solubility
• pH dependent Solubility
• Solubility in Simulated Fluids
• Formulation Stability Studies
• LogD measurements